GHK‑Cu — Subcutaneous Dosage Guide for Skin Tightening

This article is intended as an educational, guidance only. It does not replace medical advice. Always consult a qualified healthcare professional before using peptides.

Quick summary

Recommended subcutaneous dose for skin tightening: 1–2 mg per day (or every other day). Typical cycle: 3–4 weeks on, then a break. Reconstitute the 50 mg vial with 5 mL of BAC water to reach a concentration of 10 mg/mL.

What you'll need

  • GHK-Cu peptide vial (50 mg)
  • Bacteriostatic water (BAC water)
  • Insulin syringes (29–31 gauge, U-100; 0.3–1 mL)
  • Alcohol wipes, cotton pads, sharps container

Step 1 — Reconstitution

Reconstitute the 50 mg vial with 5 mL BAC water to achieve a concentration of 10 mg/mL. Clean vial tops with alcohol, slowly add the water down the vial wall, and gently swirl until dissolved. Do not shake.

Step 2 — Dosage Calculations

At 10 mg/mL concentration:

  • 1 mg dose = 0.10 mL (10 IU on an insulin syringe)
  • 2 mg dose = 0.20 mL (20 IU)

Step 3 — Subcutaneous Injection

  1. Wash hands and clean the injection site with an alcohol wipe (stomach, thigh, or love handle).
  2. Pinch a 1–2 cm fold of skin.
  3. Insert the insulin syringe at a 45–90° angle into the pinched skin.
  4. Inject the calculated volume slowly, wait 2–3 seconds, then withdraw the needle.
  5. Dispose of the needle in a sharps container and store the reconstituted vial in the fridge (2–8°C).

Suggested protocol

Dose: 1–2 mg per day (or every other day).
Cycle: 3–4 weeks on, then a break.
Time to notice effects: 4–8 weeks of consistent use.

Important safety & storage notes
  • Store reconstituted peptide refrigerated (2–8°C). Use within 20–30 days.
  • Never share or reuse needles; always use sterile technique.
  • Start at the lower end (1 mg) to assess tolerance before increasing.
  • Educational purposes only; administer under professional guidance.

Printable Quick Reference

50 mg vial → add 5 mL BAC water → 10 mg/mL
Dose conversions:
 • 1 mg = 0.10 mL = 10 IU
 • 2 mg = 0.20 mL = 20 IU
Doses per vial (approx.):
 • 50 mg (5 mL) → 50 doses @1 mg / 25 doses @2 mg

Disclaimer: Educational content only. Consult a clinician before use. Verify vendor-specific storage and handling instructions.

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